Friday, July 31, 2009

Beautiful bacteria!

This is an image of a gorgeous sulfurous spring at one of my field sites, note the purple sulfur (unconfirmed, possibly purple non-sulfur) bacteria in the center. The image is about 10 cm widthwise.

I used an inoculum of these bacteria for a Winogradsky column I started a month ago, they are growing beautifully - stay tuned for more photos!

Wednesday, July 8, 2009

Taming the fastidious organism

Biogeomicro term of the day: Fastidious organism

"An organism that has complex nutritional requirements"
ref: http://www.biology-online.org/dictionary/Fastidious_organism

Here is an open access paper using this term:
Degnan AJ, Sonzogni WC & Standridge JH (2003) Development of a Plating Medium for Selection of Helicobacter pylori from Water Samples. Appl. Environ. Microbiol. 69(5): 2914-2918
http://aem.asm.org/cgi/reprint/69/5/2914


Though I don't study human pathogens, I do work with organisms that are difficult to isolate from their more persistent "weedy" companions. The following quote from this paper seemed to sum up the challenge particularly well:

developing an acceptable selective medium for H. pylori in water presented the classical microbiological problem of finding a medium that is nutritionally rich enough to resuscitate and grow a fastidious organism while managing to inhibit the growth of all the other organisms found in water samples.


This sentence really sums up the focus of my quest for this week!